nanopore

Direct observation of DNA knots using a solid-state nanopore

Solid-state nanopores can directly observe individual knots in both linear and circular single DNA molecules of arbitrary length.

DNA nanopore translocation in glutamate solutions

Nanopore experiments have traditionally been carried out with chloride-based solutions. Here we introduce silver/silver-glutamate-based electrochemistry as an alternative, and study the viscosity, conductivity, and nanopore translocation …

Detection of Individual Proteins Bound along DNA Using Solid-State Nanopores

DNA in cells is heavily covered with all types of proteins that regulate its genetic activity. Detection of DNA-bound proteins is a challenge that is well suited to solid-state nanopores as they provide a linear readout of the DNA and DNA–protein …

Data analysis methods for solid-state nanopores

We describe a number of techniques for the analysis of solid-state nanopore ionic current traces and introduce a new package of Matlab analysis scripts with GUI front ends. We discuss methods for the detection of the local baseline and propose a new …

Velocity of DNA during translocation through a solid state nanopore

While understanding translocation of DNA through a solid-state nanopore is vital for exploiting its potential for sensing and sequencing at the single-molecule level, surprisingly little is known about the dynamics of the propagation of DNA through …

Ionic Permeability and Mechanical Properties of DNA Origami Nanoplates on Solid-State Nanopores

While DNA origami is a popular and versatile platform, its structural properties are still poorly understood. In this study we use solid-state nanopores to investigate the ionic permeability and mechanical properties of DNA origami nanoplates. DNA …

Fast Translocation of Proteins through Solid State Nanopores

Measurements on protein translocation through solid-state nanopores reveal anomalous (non-Smoluchowski) transport behavior, as evidenced by extremely low detected event rates; that is, the capture rates are orders of magnitude smaller than what is …

Non-equilibrium folding of individual DNA molecules recaptured up to 1000 times in a solid state nanopore

We investigate translocation of linear and circular double-stranded DNA molecules through solid state nanopores where each molecule is recaptured and re-translocated many times. Single molecules can be recaptured by switching voltage polarity for …